Mukhtar Ahmad, Usama Shouket, Muhammad Rizwan, Numera Saif, Hafiz Aun Muhammad and Komal Sarwar
SUMMARY
The objective of this study was to determine the physiological and biochemical reactions of Pangasius pangasius grown in a biofloc technology (BFT)-based aquaculture system. The setup time of the experiment took 90 days, and the fish were randomly split and placed into two biofloc treatment tanks (T1, T2) and one control tank. Growth, survival, hematology, and serum biochemistry performances were assessed. The results indicated that biofloc growth rearing was a better way to measure fish growth performance. After 90 days, the biofloc rearing treatment 2 (T2) had the highest absolute growth rate at 449.0 g, while treatment 1 (T1) had 439.8 g, and the control had 412.2 g. The survival rate was higher in biofloc systems (T1: 93%; T2: 91.7%) than in the control group (89.5%). Hematological tests indicated increased red blood cell (RBC) and hemoglobin levels in the biofloc groups, suggesting enhanced oxygen transfer and reduced stress. The substantial decrease in white blood cell (WBC) counts in T2 indicates improved immune function. The serum biochemistry revealed diminished glucose levels (signifying reduced stress) and elevated total protein and albumin levels in fish cultivated in bioflocs, indicating enhanced nutrient absorption. The study shows that biofloc technology improves the growth, health, and feed conversion efficiency of P. pangasius, even when external feed is scarce. This makes fish farming more sustainable and productive.
Keywords: Pangasius pangasius, Biofloc technology, Hematology, Serum biochemistry
Citation: Ahmad, M., U. Shouket, M. Rizwan, N. Saif, H. A. Muhammad, and K. Sarwar. 2026. Physiological and Haemato-Biochemical Evaluation of Pangasius pangasius Reared in a Biofloc-Based Aquaculture System. Journal of Wildlife and Ecology. 10: 14-24. doi.org/10.66470/10102